“Hyperspectral imaging of H&E stained tissue sections using a spectrally programmable light engine.”
Note: the following is a brief summary of the subject matter contained in the paper published under “6441-09, Session 1” of Photonics West Conference 6441, under the title quoted above. For a more accurate and detailed representation, please consult the paper itself.
Researchers at the Cancer Imaging Department of the BC Cancer Agency, Vancouver, BC, produced a series of hyperspectral transmission images of hematoxylin and eosin stained tissue sections from cervical biopsies, images of maximum contrast were achieved via custom software that provided extraction of spectra at each pixel. This allowed for determination of selected features and differentiation of tissue features.
Using a spectrally and temporally programmable light engine, illumination profiles were achieved via a spatial light modulator – dynamically creating any narrow or broadband spectral profile. This allowed for the selection of particular illumination wavelengths. Images were captured by a monochrome CCD camera, with several methods employed to combine images from individual and composite spectral bands to recreate color images for pathologist review.
Of note, the light engine provided for digital control of all illumination parameters – integrating the lamp, wavelength control, intensity control and exposure control in a Micro Electro Mechanical Structure (or MEMS) based system.
BCCA researchers also captured hyperspectral Images of cervical tissue sections stained with molecular specific stains and hematoxylin and processed them, using spectral unmixing techniques to enhance contrast between tissues labeled with the molecular stain and surrounding tissues.
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